HepG2 is a perpetual cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian male who had a well-differentiated hepatocellular carcinoma. Hepatocellular carcinoma is, worldwide, the fifth most-common cancer. The morphology of HepG2 cells is epithelial and they have 55 chromosome pairs. HepG2 cells can be grown successfully at a large scale, and secret many plasma proteins, such as transferring, fibrinogen, plasminogen and albumin. They can be stimulated with human growth hormone. HepG2 are adherent, epithelial-like cells growing as monolayers and in small aggregates. Not tumorigenic in immunocompromised mice.
HepG2 transfection kit is commercially available from Altogen Biosystems
Cytogenetics: human hyperdiploid karyotype – 52(47-54)<2n>XY, +2, +14, +17, +20, +2mar, t(1;21) (p22.2;p11-12), i(17q)/der(17)t(17;17)(p11;q11)
- Use Eagle’s Minimum Essential Medium (DMEM and RPMI1640 also works well) supplemented with 10% FBS. Discard culture medium every 2-3 days
- To passage cells, briefly rinse cell monolayer with 1xPBS twice and add pre-warmed (37°C) 0.05% Trypsin-EDTA solution for 5 – 7 minutes
- Once cell layer is dispersed (5-7 min at 37°C) deactivate Trypsin by adding equal volume of complete growth medium with 10% FBS
- To avoid clumping don’t agitate the cells by shaking the flask while waiting for the cells to detach
- Split cells 1:4 every 3 days (recommended), or 1:8 every 6 days.
- Cultures should be incubated at 37°C in humidified atmoshere with 5% CO2
Stable Cell Line Generation:
HepG2 cells have been demonstrated to be Neomycin G418 resistant (400 µg/mL). Development of HepG2 stable cell line services provided by Altogen Labs CRO